The preliminary stages of the 2010 Copa Sudamericana de Clubes are:
- First Stage, contested by 16 teams in 8 two-legged ties. The winner of each tie will advance to the Second Stage.
- Second Stage, contested by 30 teams, 8 of whom advanced from the First Stage, in 15 two-legged ties. The winner of each tie will advance to the Round of 16, joining the defending champion, LDU Quito.
First stage
edit| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg |
|---|---|---|---|---|
| Colo-Colo | 3–3 (a) | Universitario | 0–2 | 3–1 |
| River Plate | 4–4 (a) | Guaraní | 0–2 | 4–2 |
| Barcelona | 5–2 | Universidad César Vallejo | 2–1 | 3–1 |
| Trujillanos | 2–5 | Atlético Huila | 1–4 | 1–1 |
| Oriente Petrolero | 3–2 | Universidad de Chile | 2–2 | 1–0 |
| Olimpia | 1–3 | Defensor Sporting | 0–2 | 1–1 |
| Universidad San Martín | (a) 3–3 | Deportivo Quito | 2–3 | 2–1 |
| Santa Fe | 4–2 | Deportivo Lara | 0–2 | 4–0 |
Match A
edit| Universitario | 2–0 | Colo-Colo |
|---|---|---|
| Galindo 58' Lima 80' |
Report |
| Colo-Colo | 3–1 | Universitario |
|---|---|---|
| Cámpora 38', 52' Fuenzalida 67' |
Report | Galindo 45' |
Colo-Colo 3–3 Universitario on points. Universitario advanced on away goals.
Match B
edit| Guaraní | 2–0 | River Plate |
|---|---|---|
| Fabbro 67' Teixeira 73' |
Report |
| River Plate | 4–2 | Guaraní |
|---|---|---|
| Ramírez 23' Zambrana 66' Correa 74', 86' |
Report | Teixeira 9' Joel Benítez 45' |
River Plate 3–3 Guaraní on points. Guaraní advanced on away goals.
Match C
edit| Universidad César Vallejo | 1–2 | Barcelona |
|---|---|---|
| H. Hurtado 20' | Report | J. Hurtado 22' Noir 64' |
Barcelona advanced on points 6–0.
Match D
edit| Atlético Huila | 4–1 | Trujillanos |
|---|---|---|
| Carbonero 5' Castillo 32' Martínez 38' (pen.) Velásquez 90+1' |
Report | R. García 19' |
| Trujillanos | 1–1 | Atlético Huila |
|---|---|---|
| R. García 3' | Report | Rueda 8' |
Atlético Huila advanced on points 4–1.
Match E
edit| Universidad de Chile | 2–2 | Oriente Petrolero |
|---|---|---|
| Bueno 49' Puch 70' |
Report | Rea 46' Saucedo 48' |
Oriente Petrolero advanced on points 4–1.
Match F
edit| Defensor Sporting | 2–0 | Olimpia |
|---|---|---|
| Mora 37', 71' | Report |
| Olimpia | 1–1 | Defensor Sporting |
|---|---|---|
| Ferreyra 86' | Report | Mora 50' |
Defensor Sporting advanced on points 4–1.
Match G
edit| Deportivo Quito | 3–2 | Universidad San Martín |
|---|---|---|
| Mina 23' Fernández 61' Checa 90' |
Report | Vitti 51' Alemanno 74' |
| Universidad San Martín | 2–1 | Deportivo Quito |
|---|---|---|
| Arriola 78' (pen.) Alemanno 85' |
Report | Checa 45' |
Universidad San Martín 3–3 Deportivo Quito on points. Universidad San Martín advanced on away goals.
Match H
edit| Deportivo Lara | 2–0 | Santa Fe |
|---|---|---|
| Ocanto 13' Chalar 45' |
Report |
| Santa Fe | 4–0 | Deportivo Lara |
|---|---|---|
| Otálvaro 6' Bernal 50' Noguera 72' Anchico 90+4' (pen.) |
Report |
Santa Fe 3–3 Deportivo Lara on points. Santa Fe advanced on goal difference.
Second stage
edit| Team 1 | Agg. | Team 2 | 1st leg | 2nd leg |
|---|---|---|---|---|
| San José | 5–1 | Atlético Huila | 1–1 | 4–0 |
| Argentinos Juniors | 1–2 | Independiente | 0–1 | 1–1 |
| Peñarol | 3–1 | Barcelona | 1–0 | 2–1 |
| Palmeiras | 3–2 | Vitória | 0–2 | 3–0 |
| Caracas | 1–2 | Santa Fe | 1–2 | 0–0 |
| Avaí | 3–2 | Santos | 3–1 | 0–1 |
| Deportes Tolima | 2–1 | Oriente Petrolero | 0–1 | 2–0 |
| Unión San Felipe | 2–2 (8–7 p) | Guaraní | 1–1 | 1–1 |
| Banfield | 2–1 | Vélez Sársfield | 1–0 | 1–1 |
| Emelec | 6–2 | Universidad San Martín | 1–2 | 5–0 |
| Atlético Mineiro | 1–0 | Grêmio Prudente | 0–0 | 1–0 |
| Cerro Porteño | 2–3 | Universitario | 0–1 | 2–2 |
| Grêmio | 1–3 | Goiás | 1–1 | 0–2 |
| Sport Huancayo | 2–9 | Defensor Sporting | 0–9 | 2–0 |
| Estudiantes | 1–2 | Newell's Old Boys | 0–1 | 1–1 |
Match O1
edit| Atlético Huila | 1–1 | San José |
|---|---|---|
| Martínez 61' (pen.) | Report | de Souza 27' (pen.) |
| San José | 4–0 | Atlético Huila |
|---|---|---|
| Villalba 5' de Souza 23' Bejarano 42' Medina 66' |
Report |
San José advanced on points 4-1.
Match O2
edit| Argentinos Juniors | 1–1 | Independiente |
|---|---|---|
| Ortigoza 34' (pen.) | Report | Gracián 66' |
Independiente advanced on points 4–1.
Match O3
edit| Peñarol | 2–1 | Barcelona |
|---|---|---|
| Ramis 72' Pacheco 90+4' (pen.) |
Report | S. Sosa 79' (o.g.) |
Peñarol advanced on points 6–0.
Match O4
edit| Palmeiras | 3–0 | Vitória |
|---|---|---|
| Tadeu 45', 58' Marcos Assunção 89' |
Report |
Palmeiras 3–3 Vitória on points. Palmeiras advanced on goal difference.
Match O5
edit| Caracas | 0–0 | Santa Fe |
|---|---|---|
| Report |
Santa Fe advanced on points 4–1.
Match O6
edit| Avaí | 0–1 | Santos |
|---|---|---|
| Report | Zé Eduardo 24' |
Avaí 3–3 Santos on points. Avaí advanced on goal difference.
Match O7
edit| Deportes Tolima | 2–0 | Oriente Petrolero |
|---|---|---|
| Medina 2' Perlaza 25' |
Report |
Deportes Tolima 3–3 Oriente Petrolero on points. Deportes Tolima advanced on goal difference.
Match O9
editUnión San Felipe 3–3 Guaraní on points. Unión San Felipe advanced 8–7 on penalties.
Match O10
edit| Vélez Sársfield | 0–1 | Banfield |
|---|---|---|
| Report | García 30' |
| Banfield | 1–1 | Vélez Sársfield |
|---|---|---|
| Carrusca 15' | Report | Cristaldo 90' |
Banfield advanced on points 4–1.
Match O11
edit| Universidad San Martín | 2–1 | Emelec |
|---|---|---|
| Vitti 45' Quinteros 74' |
Report | Rojas 4' |
| Emelec | 5–0 | Universidad San Martín |
|---|---|---|
| Ayoví 12', 61' Quiroz 17' Giménez 46' Valencia 89' |
Report |
Emelec 3–3 Universidad San Martín on points. Emelec advanced on goal difference.
Match O12
edit| Atlético Mineiro | 1–0 | Grêmio Prudente |
|---|---|---|
| Ricardinho 90+2' | Report |
Atlético Mineiro advanced on points 4–1.
Match O13
edit| Universitario | 1–0 | Cerro Porteño |
|---|---|---|
| Galindo 83' | Report |
| Cerro Porteño | 2–2 | Universitario |
|---|---|---|
| dos Santos 54' Núñez 73' (pen.) |
Report | Galindo 23' Cirillo 68' |
Universitario advanced on points 4–1.
Match O14
edit| Grêmio | 0–2 | Goiás |
|---|---|---|
| Report | Amaral 9' Éverton Santos 90' |
Goiás advanced on points 4–1.
Match O15
edit| Defensor Sporting | 9–0 | Sport Huancayo |
|---|---|---|
| de Souza 1' (pen.), 46' Risso 43' Amado 47' Mora 56', 66' (pen.) Luna 61' (pen.), 79' Aranda 76' |
Report |
Note: This is the largest margin of victory in the history of the Copa Sudamericana.
| Sport Huancayo | 2–0 | Defensor Sporting |
|---|---|---|
| Suárez 45' (o.g.) Ramírez 66' |
Report |
Sport Huancayo 3–3 Defensor Sporting on points. Defensor Sporting advanced on goal difference.
Match O16
edit| Newell's Old Boys | 1–0 | Estudiantes |
|---|---|---|
| Formica 90+3' (pen.) | Report |
| Estudiantes | 1–1 | Newell's Old Boys |
|---|---|---|
| Fernández 12' | Report | Borghello 43' |
Newell's Old Boys advanced on points 4–1.