Sodium/glucose cotransporter 1
Template:PBB Sodium/glucose cotransporter 1 is a protein that in humans is encoded by the SLC5A1 gene.[1][2]
Cloning of the sodium-glucose cotransporter SGLT1
Co-transport proteins of mammalian cell membranes had eluded efforts of purification with classical biochemical methods until the late 1980s. These proteins had proven difficult to isolate because they contain hydrophilic and hydrophobic sequences and exist in membranes only in very low abundance (<0.2% of membrane proteins). The rabbit form of SGLT1 is the first mammalian co-transport protein ever to be cloned and sequenced, and this scientific break-through was reported in 1987. To circumvent the difficulties with traditional isolation methods, Swiss-born biochemist Matthias Hediger and his collaborators at UCLA used a novel technique of expression cloning. They size-fractionated large amounts of rabbit intestinal mRNA with a preparative gel electrophoresis device developed by Hediger. These size fractions were then sequentially injected into Xenopus oocytes to ultimately find the RNA species that induced the expression of sodium-glucose cotransport.[3]
See also
- Solute carrier family
- SGLT Family
- SGLT2
Interactions
SLC5A1 has been shown to interact with PAWR.[4]
References
- ^ Turk E, Martin MG, Wright EM (1994). "Structure of the human Na+/glucose cotransporter gene SGLT1". J Biol Chem. 269 (21): 15204–9. PMID 8195156.
{{cite journal}}: Unknown parameter|month=ignored (help)CS1 maint: multiple names: authors list (link) - ^ "Entrez Gene: SLC5A1 solute carrier family 5 (sodium/glucose cotransporter), member 1".
- ^ Hediger MA, Coady MJ, Ikeda TS, Wright EM (1987). "Expression cloning and cDNA sequencing of the Na+/glucose co-transporter". Nature. 330 (6146): 379–81. doi:10.1038/330379a0. PMID 2446136.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Xie, Jun (2004). "Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane". J. Biol. Chem. 279 (27). United States: 28266–75. doi:10.1074/jbc.M401495200. ISSN 0021-9258. PMID 15090548.
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Further reading
This article incorporates text from the United States National Library of Medicine, which is in the public domain.
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