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Join a webinar today hosted by the BRAIN Initiative Cell Atlas Network (BICAN) at 10 am PDT! BICAN and the BRAIN Initiative Cell Census Network (BICCN) recently published a new collection of studies focused on defining and characterizing the underlying gene expression, gene regulatory, and morphoelectric features of cell types in the human and non-human primate (NHP) brain. Today's webinar dives into these publications and explores the different technologies such as single cell spatial genomics leveraged during the research. Speakers: -Ed Lein, Senior Investigator at the Allen Institute for Brain Science: Introduction -Kimberly Siletti, University Medical Center Utrecht, Netherlands: Transcriptomic diversity of cell types in the adult human brain -Wei Tian, Salk Institute: Epigenomic complexity of the human brain revealed by single-cell DNA methylomes and 3D genome structures -Trygve Bakken, Allen Institute for Brain Science: Comparative transcriptomics reveals human-specific cortical features -Chang Kim, University of California, San Francisco: Spatiotemporal molecular dynamics of the developing human thalamus -Lijuan Liu, Southeast University, China: Whole Human-Brain Mapping of Single Cortical Neurons for Profiling Morphological Diversity and Stereotypy Register here: https://hubs.ly/Q026N1sQ0 #NIH #BICAN #BICCN #Webinar #Neuroscience #SingleCell #SpatialGenomics #MERFISH #HumanBrain #BrainScience #ScienceMagazine #ScienceAdvances #ScienceTranslationalMedicine

Join the quest to map the mouse brain! Scientists around the globe are using a combination of single-cell sequencing and spatial transcriptomics to uncover the intricate cellular diversity of the mouse brain. These methods such as MERFISH allow researchers to create detailed atlases of various cell types and their gene expressions within different brain regions. Read the latest technology feature in {{linkedin_mention(urn:li:organization:7131|Nature Portfolio)}} to learn how MERFISH and other techniques enable scientists to explore the brain and gain deeper insights into cellular interactions, molecular mechanisms, and potential therapeutic opportunities: https://lnkd.in/eYFHTUwN #MERFISH #MouseBrain #Neuroscience #BrainScience #SingelCell #SpatialTranscriptomics #SpatialGenomics #CellAtlas #BrainMapping

🔬🧬 Recent Breakthrough in Aging Research🌐 Recent studies have shown a remarkable discovery in understanding aging in mammals. A dedicated team of researchers has identified a critical neuronal subpopulation in the dorsomedial hypothalamus (DMH), marked by Ppp1r17 expression. These DMHPpp1r17 neurons play a pivotal role in regulating aging and longevity in mice. The team discovered that DMHPpp1r17 neurons regulate physical activity and white adipose tissue (WAT) function. This includes the secretion of extracellular nicotinamide phosphoribosyltransferase (eNAMPT) through sympathetic nervous stimulation. A key aspect of their research highlights the phosphorylation and nuclear-cytoplasmic translocation of Ppp1r17, which is regulated by cGMP-dependent protein kinase G (PKG; Prkg1). This process is crucial for gene expression regulation of synaptic function, impacting synaptic transmission and WAT function. Both DMH-specific Prkg1 knockdown, which curtails age-associated Ppp1r17 translocation, and the chemogenetic activation of DMHPpp1r17 neurons have shown promising results. These interventions ameliorated age-associated dysfunction in WAT, increased physical activity, and extended lifespan in their models. This groundbreaking work demonstrates the vital role of inter-tissue communication between the hypothalamus and WAT in controlling mammalian aging and longevity. link: https://lnkd.in/gBhHP9tB #AgingResearch #Neuroscience #Longevity #InnovativeScience #HealthcareBreakthrough

Recent progress in chemistry, molecular and cell biology, and nanotechnology has expanded the strength of #MRI to characterize pathological tissue changes by providing tools that visualize cellular and molecular processes. A wealth of novel imaging probes, reporter gene - and cell-labelling strategies provide the basis for gaining unprecedented insight into the pathomechanisms of neurological disorders. Here, we illustrate examples from academic research groups’ work on the application of molecular and cellular MRI in models of neurological disorders and its use for the evaluation of gene and cell therapies. Download the paper here: https://lnkd.in/dt_E28yN Please visit us at booth C08 at the ISMRM Conference on May 4-9 in Singapore to learn more about Bruker's MRI solutions: https://lnkd.in/dwggq4q7 #ISMRM #ISMRM2024 #Imaging #PreclinicalImaging #Neurology

How can direct brain stimulation reshape our approach to neurological disorders? A study from the Chinese Academy of Sciences used our #Starstim device to look into how intracranial alternating current stimulation (#iACS) on rats can influence gene expression and cellular diversity in the brain. 🐀🧠 🔍 The team used Starstim to apply a 40 Hz current to the rat's brain, tracking the changes in neuronal populations and gene expression, focusing on the Rgs9 gene, known for its role in dopaminergic signaling. This approach unveiled potential new pathways for treating neurological disorders by enhancing neurogenesis and #neuron sensitivity. Want to learn more about how they achieved this? Check out the full #research study here: https://lnkd.in/dc9VWDHG 📄 Congratulations to Yan Wang, Yongchao Ma, Qiuling Zhong, Bing Song, and Ming Qian from the Shenzhen Institutes of Advanced Technology! Your work is paving the way for future innovations in brain health treatments. 👏 #Medtech #Electroceuticals #Technology #Healthtech #Healthcare #Science #Innovation #Study #RNA #ResearchArticle #Neuroscience #Neurotechnology #Starstim #tDCS #BrainStimulation #tACS #tES #Neurotech SHENZHEN INSTITUTE OF ADVANCED TECHNOLOGY CHINESE ACADEMY OF SCIENCES

C9ORF72 and SOD1 ALS: Unveiling Cellular Mechanisms and Expanding Therapeutic Horizons In two studies from the Evangelos Kiskinis group, researchers delved into the pathological cascade of ALS. In the first study, published in Science Advances https://lnkd.in/efJ8HYYw, they demonstrated that C9ORF72 aggregates have a stronger binding affinity for ribosomal RNA (rRNA) than ribosomal proteins. Moreover, employing an rRNA bait mitigated poly-GR toxicity in patient neurons and Drosophila models. In the second study, published in Cell Reports https://lnkd.in/eig4-8vX, the researchers employed proteomics to investigate proteome-wide degradation dynamics in SOD1 mutant (mSOD1) iPSC motor neurons. They identified that ALS-causal valosin-containing protein (VCP) degrades slower in mSOD1 cells compared to controls. VCP exhibits an altered interactome and can modulate the degradation of mutSOD1. Furthermore, VCP overexpression successfully rescued mutSOD1 toxicity in vitro and in a C. elegans model. https://lnkd.in/efFhjm5N #genetics #genomics #precisionmedicine #genomicmedicine #brain #neurology #neuroscience #neurodegeneration #als #omics #transcriptomics #proteomics #therapeutics #neurons #biomarkers #translationalresearch #biotechnology #innovation #research #science #sciencecommunication

ioCRISPR-Ready Cells™ allow you to harness knockout experiments and screening capabilities very, very quickly.These iPSC-derived cells can be used with viral and sgRNA modalities! Get in touch if you'd like to learn more, we also have excellent technical support if you need help designing the screening experiment! #crispr #crisprcas9 #ipsc #neuroscience #screening #knockout

GBA1 beyond the lysosome, Gaucher, and Parkinson's: discovery of brain non-lysosomal transcripts coded by the GBA1 gene The GBA1 gene, mutated in Gaucher disease and a risk factor for Parkinson's, proved difficult to study due to its highly similar pseudogene, GBAP1. Long-read RNA sequencing of human brains revealed significant differences in gene expression compared to traditional methods. This identified previously unknown transcripts from both genes, including protein-coding ones in the brain with functions beyond the known role of GBA1. Analyzing brain cell types further showed regional variations in these transcripts. These findings suggest new, non-lysosomal roles for both GBA1 and GBAP1, potentially impacting our understanding of GBA1's function in health and disease. The article was published in Science Advances: https://lnkd.in/eP82Wii8. #genetics #genomics #precisionmedicine #genomicmedicine #raredisease #gaucher #parkinson #brain #neurology #neuroscience #neurodegeneration #rna #lysosome #biomarkers #therapeutics #biotechnology #innovation #research #science #sciencecommunication

Recent findings have set a new trajectory in our understanding of schizophrenia (SCZ) by pinpointing the role of microRNA137 (miR137) and its host gene MIR137, which genetic variations have been associated with a heightened SCZ risk. Scientists have now adventurously tapped into nanotechnology, using LNPs to deliver therapeutic RNA aimed at potentially rectifying synaptic dysfunction linked with SCZ at a molecular level. The team tested LNPs composed of MC3 (ionizable) only, SM102 (ionizable cationic) only, SM102 and 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) (cationic), or MC3 and DOTAP lipids, and found that LNP formulations combining MC3 and DOTAP lipids were the most efficient at cargo release. 📌 Key Findings: 1) LNPs were able to effectively release miR137 and inhibit target transcripts in neuroblastoma cells. 2) Biodistribution studies revealed that LNPs containing firefly luciferase mRNA remained localized in the mouse prefrontal cortex (PFC), avoiding off-target organ circulation. 3) LNPs with Cre mRNA exhibited preferential co-expression in neuronal cells over microglial or astrocytic cells. 4) miR137 influenced glutamatergic synaptic protein networks, commonly dysregulated in SCZ, as per quantitative proteomics findings. Navigating the complex landscape of miR137 and its implication in glutamatergic synaptic functionality opens an enthralling chapter for SCZ research and potential therapies. The use of LNPs as RNA delivery vehicles, especially with their capability to remain localized and thus, minimize off-target effects, is compelling. But the leap from cellular and murine models to potential clinical applications bears multifaceted challenges and ethical considerations. The evolving spectrum of LNP applicability in brain-specific RNA therapeutic delivery is poised to be a fascinating journey, interweaving the threads of genomics, neurobiology, and nanotechnology. 📚 If you want to know more, check: https://lnkd.in/e3gnmFir #Neurobiology #Nanotechnology #RNAtherapy #Genomics #miR137 #Biotechnology #Neuroscience #LNPs #lipidnanoparticles

🚀 Breaking New Ground in Spatial Multi-Omics! 🧬 Exciting advancements in #Biotechnology! A team led by Xiaofeng Wu and colleagues has developed MiP-seq (multi-omics in situ pairwise sequencing), a revolutionary method for detecting DNAs, RNAs, proteins, and biomolecules at subcellular resolution. 🌟 Key Highlights: - Enhanced Decoding Capacity: Compared to other methods, MiP-seq boosts decoding capacity while slashing sequencing and imaging costs. 📉 - Comprehensive Detection: Effectively detects gene mutations, allele-specific expression, and RNA modifications. 🧬 - Integration with Advanced Imaging: Compatible with in vivo calcium imaging and Raman imaging, enabling the creation of a spatial multi-omics atlas of mouse brain tissues. 🧠 - Innovative Dilution Strategy: Resolves optically crowded signals during in situ sequencing. 🎨 This high-throughput approach is set to revolutionise the multidimensional analysis of molecular and functional maps of tissues, paving the way for groundbreaking discoveries in cellular functions and disease mechanisms. 🔬 #MultiOmics #MiPseq #BiotechInnovation #SpatialOmics #Genomics #Proteomics #Neuroscience #BiomedicalEngineering #ResearchBreakthrough #ScienceInnovation