LifeCanvas Technologies Inc.’s Post

Love it! In neuroscience ,at the end of the day, it’s all about addressing the question of spatial and temporal resolution, which is the most critical point to understand the most complex material in this universe, the 🧠. How deep you can see ,how localized you can focus and how specific you can target, there is always space to grow!!!

🔬 Exciting News from LifeCanvas Technologies! We are excited to share the latest publication in Science, featuring groundbreaking research from the Chung Lab at Massachusetts Institute of Technology. This research, led by Kwanghun Chung, our founder, includes significant contributions from Jeffrey Stirman (CTO at LifeCanvas Technologies Inc.) and three former Chung Lab members who are now part of LifeCanvas Technologies Inc.: Ji Wang (Director of Mechanical Products), Nicholas Evans (Senior Microscope Engineer), and Webster Guan (Principal Image Data Scientist). 🧠 The paper showcases how LifeCanvas' cutting-edge technologies, including #MEGAtome, #SHIELD, and #MegaSPIM, are transforming the study of human organs, particularly the brain. - #SHIELD: Ensures permanent protein preservation through multiple imaging cycles. - #MEGAtome: Precision slicing for maintaining critical structural details in large biological samples. - #MegaSPIM: Captures multiscale data with high-throughput cellular resolution and adaptability for large tissue samples. These technologies have been pivotal in analyzing #Alzheimer's disease pathology in human brains, revealing detailed insights into cell distributions, neuronal structures, and synaptic connections. This holistic mapping approach opens new avenues for understanding complex brain disorders. ➡ At LifeCanvas Technologies, we’re committed to pushing the boundaries of tissue imaging and analysis. We believe this technology will significantly advance our knowledge of brain architecture and its alterations in diseases, paving the way for better diagnostics and therapies. Our goal is to empower researchers to conduct thorough, integrative analyses of human and animal brains at unmatched speed and resolution. 🔗 Full access here: https://lnkd.in/duhgpzE5

Check out the newly published Science paper utilizing MegaSPIM, MegaTome, and SHIELD for investigating Alzheimer’s disease in human brains!!

🔬 Automated #CellResearch with LIFTOSCOPE 🔬 Cell analysis and separation play a crucial role in biological processes. Until now, these steps were often performed manually, which was not only time-consuming but also involved the use of various devices. The LIFTOSCOPE prototype presents an innovative solution to these challenges. #LIFTOSCOPE combines three key functions in one system: 1️⃣ high-speed microscopy 2️⃣ image analysis 3️⃣ laser-induced forward transfer (LIFT) The prototype impressively demonstrates the feasibility of this innovative concept. Particularly fascinating is the ability to perform all three steps directly on commercially available microtiter plates without additional sample preparation. This LIFTOSCOPE concept opens up a wide range of applications, from the targeted collection of rare cells to the isolation of single cells from large colonies - all without the need for fluorescent markers. The modularity of the system also enables separate microscopy and LIFT applications. The trainability of the image analysis allows easy adaptation to new applications. The prototype represents a significant step forward in automated cell research. 💡 For more details on the prototype and the concept, please read the full paper. 👉 If you would like to learn more about this project or our research activities in the field of High-Speed-Microscopy and automated image processing, please contact our expert Florian Nienhaus. Ann-Sophie Aurich Bastian Nießing Robert Schmitt Dr. Nadine Nottrodt Richard L. Fraunhofer-Institut für Produktionstechnologie IPT Werkzeugmaschinenlabor WZL der RWTH Aachen Fraunhofer ILT #DeepLearning #automated #imageprocessing #microscopy

𝐈𝐧𝐭𝐞𝐠𝐫𝐚𝐭𝐞𝐝 𝐩𝐥𝐚𝐭𝐟𝐨𝐫𝐦 𝐟𝐨𝐫 𝐦𝐮𝐥𝐭𝐢𝐬𝐜𝐚𝐥𝐞 𝐦𝐨𝐥𝐞𝐜𝐮𝐥𝐚𝐫 𝐢𝐦𝐚𝐠𝐢𝐧𝐠 𝐚𝐧𝐝 𝐩𝐡𝐞𝐧𝐨𝐭𝐲𝐩𝐢𝐧𝐠 𝐨𝐟 𝐭𝐡𝐞 𝐡𝐮𝐦𝐚𝐧 𝐛𝐫𝐚𝐢𝐧. Understanding cellular architectures and their connectivity is essential for interrogating system function and dysfunction. However, we lack technologies for mapping the multiscale details of individual cells and their connectivity in the human organ-scale system. Here, a platform that simultaneously extracts spatial, molecular, morphological, and connectivity information of individual cells from the same human brain was developed. The platform includes three core elements: a vibrating microtome for ultraprecision slicing of large-scale tissues without losing cellular connectivity (MEGAtome), a polymer hydrogel-based tissue processing technology for multiplexed multiscale imaging of human organ-scale tissues (mELAST), and a computational pipeline for reconstructing three-dimensional connectivity across multiple brain slabs (UNSLICE). This platform was applied for analyzing human Alzheimer’s disease pathology at multiple scales and demonstrating scalable neural connectivity mapping in the human brain. https://lnkd.in/gJ25B7Mj

Molecular Devices 𝗜𝗺𝗮𝗴𝗲𝗫𝗽𝗿𝗲𝘀𝘀 𝘀𝘆𝘀𝘁𝗲𝗺𝘀 are designed with a variety of hardware (laser and LED) and software (image-based) #autofocus modes that allow you to find and preserve in focus images between experiments and minimize focus drift. The autofocus modes can accommodate a wide range of samples, culture vessel thicknesses, objectives, and imaging parameters. Tip no. 3 of 7 comes from our Cellular Imaging Applications Scientist, Matthew Hammer. Continue learning about hardware and software autofocus methods in our “Tips for running a successful #livecellimaging experiment” Lab Notes blog post: https://bit.ly/3fYOlzv. To find out more, you may like to contact us at info@its-sciencemedical.com. #MolecularDevices #microscopy #lifescience #cellbiology #science #autofocus #itssciencemedical

3D Model: This Is How Body's Building Blocks Are Made I recently came across an interesting article around a study held in Novo Nordisk Foundation Center for Protein Research by Associate Professor Eva Kummer, and it made me wonder about the horizon of applications of 3D modeling. It's beyond imangination. In a landmark study published in Nature Communications, researchers led by Eva Kummer have achieved a remarkable feat in visualizing the ribosome, the cellular powerhouse responsible for protein synthesis. Using advanced electron microscopy, they've constructed a detailed 3D model, shedding light on the intricate process of ribosome assembly. This groundbreaking research offers unprecedented insights into how over 80 different components fold, assemble, and move within the ribosome, unveiling previously unknown details about early-stage assembly. Such discoveries not only deepen our understanding of cellular function but also hold promise for addressing diseases linked to ribosome dysfunction, including neurodegenerative disorders and heart conditions. By unraveling the mysteries of cellular machinery, we're advancing scientific understanding and paving the way for innovative treatments and interventions. This milestone underscores the importance of interdisciplinary collaboration and technological innovation in driving scientific progress. #3DModeling #CellularBiology #ScientificDiscovery #Innovation #Research #LifeSciences Link to the study: https://lnkd.in/dMgNzSz8"

🔬 Commonly Used #Dyes for In Vivo #Fluorescence Imaging 🧪 In vivo imaging is a key enabling technology in biomedical research and clinical applications. It is widely used for conducting long-term studies on living animals, visualise and study the biological processes without causing any harm, providing essential data for #preclinical and #translational research. One of the key success factor for in vivo fluorescence imaging? Choosing the right dyes - ones that are brilliantly bright, long-lasting, and perfectly #biocompatible. Find out more in our latest blog post: https://lnkd.in/gyyFGyAd #InVivoImaging #Fluorescence #CellLabelling #Biomedical #Research #Luminicell Nanolumi #StemCell #InVivo #Regenerative #Medicine

📍 Integrated platform for multiscale molecular imaging and phenotyping of the human brain    Park et al. developed a platform that simultaneously extracts spatial, molecular, morphological, and connectivity information of individual cells from the same human brain.    Key points of the platform:  ✅ A vibrating microtome for ultraprecision slicing of large-scale tissues without losing cellular connectivity (MEGAtome)  ✅ A polymer hydrogel-based tissue processing technology for multiplexed multiscale imaging of human organ–scale tissues (mELAST)  ✅ A computational pipeline for reconstructing three-dimensional connectivity across multiple brain slabs (UNSLICE)  ✅ Analyzing human Alzheimer’s disease pathology at multiple scales and demonstrating scalable neural connectivity mapping in the human brain. ➡ More details: https://lnkd.in/duhgpzE5 #MassachusettsInstituteofTechnology #MIT  #spatialomics #spatialbiology #singlecellanalysis #singlecell

Organoids are not always three-dimensional constructs ! Using PEG hydrogels, researchers crafted a model of neuroinflammation perfect for imaging purposes! https://lnkd.in/dUpNYjsb 53/366