LifeCanvas Technologies Inc.’s Post

RNAscope for marker visualization in neuroscience could have been a game-changer for me if I discovered it earlier 🤦♂️ RNA visualization offers several advantages over traditional protein staining methods in multiple contexts: 📖 Example: Visualizing GABAergic neurons with GAD protein staining is usually a slippery slope of long-optimization and poor results 💡 Solution: GAD1/GAD2 RNA is abundant and localized in the soma of GABAergic neurons. RNAscope staining gives a bright and crisp signal that makes it easy to identify and segment GABAergic neurons. 📖 Example: VGLUT1 is expressed in glutamatergic neurons in the brain, mainly in the cortex and hippocampus. Antibodies work great but VGLUT1 protein is enriched in neurites, making it hard to visualize individual cells. 💡 Solution: VGLUT1 (SLC17A7) staining with RNAscope leads to a crisp, somatic staining allowing you to reliably identify glutamatergic neurons in the cortex/hippocampus. 📖 Example: CXCL12 is a crucial chemokine during brain development but protein staining leads to a diffuse signal. Same is true of inflammatory markers like IL6, IFNa, IFNg... 💡 Solution: By visualizing RNA, RNAscope allows detection of the specific cells expressing soluble factors, which can help you pinpoint the cells expressing a specific chemokine or inflammatory marker. Check-out this new brochure showing several markers in the brain that have been used with RNAscope (with studies attached for examples of staining) ! #RNAscope #Spatialbiology #Neuroscience #Neuroinflammation

📢 TissueGnostics (TG) Academy Webinar: Practical Approaches for Using Tissue Cytometry for Research Applications In the next issue of TG Academy Webinars, Dr. Henning Ulrich, University of Sao Paulo, talked about applications of tissue cytometry in neuroscience and cell biology. He presents his research on amyloid plaques and neuroinflammation in an Alzheimer’s disease APP/PS1 mouse model, and shows other examples in cell biology. Webinar Link: https://lnkd.in/dcJq6g24 👈 We invite you also to visit Schaefer Technologie GmbH website and learn more about further TG-related hardware and software solutions for your applications: https://lnkd.in/eZmUZnNK 👈 <<Plan a Demo with Your Samples!>> #technologie #imageanalysis #quantitativeanalysis #tissue #tissuemicroarrays #wholeslide #scanner #imagingsolutions  #artificialintelligence #deepneuralnetworks #deeplearning #machinelearning #appdevelopment #classification #segmentation #cancerresearch #immunohistochemistry #app #ai #morphology #characterization #imaging #Imageviewer #cellular #automationsolutions #spatialcomputing #spatialbiology #digitalpathology #spatialanalysis #spatialdata #tumormicroenvironment  #immunology  #cytometry #tissue #quantitativedata  #Video #Introduction #success #webinars #research #neurosciences #cellbiology

It isn't too late to join this hybrid masterclass, Christine Hanko has pulled together an outstanding lineup of #spatialbiology and #multiplexing experts - you won't want to miss it! #AI #imageanalysis #multiplex #phenotyping #phenoplex

#AMCDiscoveries Neuronal swelling is a pathological feature of stroke which contributes to the formation of cytotoxic edema. Under hypoxic condition, aberrant accumulation of sodium and chloride ions inside neurons increases osmotic pressure, leading to cell volume increase. Researchers from National Neuroscience Institute (NNI) and Duke-NUS Medical School examined the role of SLC26A11 in cortical neurons during hypoxia. The in vivo effect of blocking SLC26A11 under hypoxic conditions was also further studied using a rat model of stroke reperfusion. Their findings demonstrated that SLC26A11 is a major pathway for chloride entry in stroke, contributing to neuronal swelling and inhibition of SLC26A11 could be a novel therapeutic strategy for stroke. Read more in Journal of Molecular Neurobiology, Springer Nature Group: https://bit.ly/3RGl6Fk AMC researchers involved: Wei Shunhui, Bo Chen, See Wee Low, Charlene Priscilla Poore , Yahui Gao, Ping Liao #AMRInews #research #biomedical #Singapore #NNI #DukeNUS #stroke #neuronalswelling #SingHealth #AMC Visit SingHealth Duke-NUS AM Research Institute (AMRI) at bit.ly/aboutAMRI

#Neurosphere and brain #organoid researchers, check out this article on #LabAutomation with real-world examples! Learn how the #GelCount platform is enhancing lab's counting and sizing of these objects and unlocking new research possibilities. #Neurospheres #BrainOrganoids #LabAutomation #ScientificResearch #PrecisionAnalysis #SfN #ResearchInnovation #Neuroscience

#Neurospheres and brain #organoid investigators should find this useful! Read this article on #automation in this growing area with several real-world publication examples. Discover how the #GelCount platform can ramp up your lab's counting and sizing of these fascinating objects and unlock new possibilities in your research! #Neurospheres #BrainOrganoids #ScientificResearch #LabAutomation #PrecisionAnalysis #SfN #ResearchInnovation #Neuroscience

[RESEARCH] « WAVE OF DEATH »: FOLLOWING ITS PATH When brain oxygenation is cut off for a prolonged period, the electrical activity of the cerebral cortex is quickly reduced to zero. But that’s not the end of the story… Researchers at Institut du Cerveau – Paris Brain Institute, coordinated by Séverine Mahon, have shown that a “wave of death” appearing on the flat electroencephalogram is initiated deep in the cortex. It slowly spreads in this brain region until consciousness is finally extinguished, but it does not always signify permanent death. Indeed, in the event of a successful reoxygenation of the brain, the wave of death is followed by a “wave of resuscitation,” which heralds a slow recovery of brain functions. These findings, published in the journal Neurobiology of Disease, could help researchers determine which populations of neurons are most vulnerable in the event of cardiorespiratory arrest—and ultimately reduce the risk of neurological sequelae. 👇 Find the study on our website: https://lnkd.in/g-peVHh8

At positive neuroscience; when we study electromagnetic fields and radionics of life in correlation to our perceivable conscious, we can then learn about certain radio wave propagations instigating peculiar stimuli. Furthermore, the frequencies, photonic signaling and synaptic connectivity can also be observed in varying states of mind and stages of life. Studying NDEs may usher into panorama of observations and improve our perceptual orientation regarding the so called wave of death and/or pandora of neocosmic quantum consciousness.

With new BRAIN Initiative funding, the Allen Institute has launched three new projects to decipher the secrets of neurobiology. It's exciting to see how rapidly all this is advancing! (1) map the fine structures and connections in a 10mm3 piece of the mouse brain using electron microscopy (2) apply new, cutting-edge techniques known as BARseq and BRICseq to map the long-range connections of hundreds of thousands of neurons in the macaque brain (3) scale up techniques that characterize neurons by their inter-areal projections, 3D shape, electrical properties, and gene expression to better understand different types of cells across the whole mouse brain #neuroscience #connectomics #brain #neurotech #microscopy https://lnkd.in/gKP8ezSE

CULTURE WITH US. It's been 4 - 12 hours since thawing human iPSC-derived ioSensory Neurons™ and they have rapidly acquired a homogenous neuronal phenotype. No mitomycin treatment needed! https://hubs.ly/Q022zwM-0 Our simple two-step protocol uses just one medium and doesn't require any purification steps. Watch this amazing real-time footage of the neuronal development and neurite growth of precision reprogrammed ioSensory Neurons from Day 0. Explore more ioSensory Neurons data: https://hubs.ly/Q022zwM-0 Images were captured every 30 mins for 8 hours using Nanolive SA's automated label-free, high-content live cell imaging platform. #research #DrugDiscovery #pain #ChronicPain #ScienceCommunication #optiox #ioCells #sensory #SensoryNeurons #iPSC #StemCells #neuroscience